Antibodies Present in Normal Human Serum Inhibit Invasion of Human Brain Microvascular Endothelial Cells byListeria monocytogenes
Open Access
- 1 January 2003
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (1) , 95-100
- https://doi.org/10.1128/iai.71.1.95-100.2003
Abstract
Listeria monocytogenes causes meningitis and encephalitis in humans and crosses the blood-brain barrier by yet unknown mechanisms. The interaction of the bacteria with different types of endothelial cells was recently analyzed, and it was shown that invasion into, but not adhesion to, human brain microvascular endothelial cells (HBMEC) depends on the product of the inlB gene, the surface molecule InlB, which is a member of the internalin multigene family. In the present study we analyzed the role of the medium composition in the interaction of L. monocytogenes with HBMEC, and we show that invasion of HBMEC is strongly inhibited in the presence of adult human serum. The strong inhibitory activity, which is not present in fetal calf serum, does not inhibit uptake by macrophage-like J774 cells but does also inhibit invasion of Caco-2 epithelial cells. The inhibitory component of human serum was identified as being associated with L. monocytogenes-specific antibodies present in the human serum. Human newborn serum (cord serum) shows only a weak inhibitory activity on the invasion of HBMEC by L. monocytogenes.Keywords
This publication has 25 references indexed in Scilit:
- Comparative Genomics of Listeria SpeciesScience, 2001
- ListeriaPathogenesis and Molecular Virulence DeterminantsClinical Microbiology Reviews, 2001
- Antibodies against Listerial Protein 60 Act as an Opsonin for Phagocytosis ofListeria monocytogenesby Human Dendritic CellsInfection and Immunity, 2001
- Listeria monocytogenes-Infected Phagocytes Can Initiate Central Nervous System Infection in MiceInfection and Immunity, 2001
- Interaction of Listeria monocytogenes with Human Brain Microvascular Endothelial Cells: an Electron Microscopic StudyInfection and Immunity, 2000
- Listeria monocytogenes-infected human umbilical vein endothelial cells: internalin-independent invasion, intracellular growth, movement, and host cell responsesFEMS Microbiology Letters, 1997
- Bacteremia is required for invasion of the murine central nervous system by Listeria monocytogenesMicrobial Pathogenesis, 1995
- Immunity to Intracellular BacteriaAnnual Review of Immunology, 1993
- Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocciCell, 1991
- Demonstration of Opsonic and Protective Activity of Human Cord Sera against Type III Group B Streptococcus that Are Independent of Type-Specific AntibodyPediatric Research, 1988