Expression of the tumor necrosis factor locus is not necessary for the cytolytic activity of T lymphocytes.
Open Access
- 15 March 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 140 (6) , 1916-1922
- https://doi.org/10.4049/jimmunol.140.6.1916
Abstract
In order to test whether tumor necrosis factors alpha (TNF-alpha) or beta (TNF-beta, also known as lymphotoxin) are involved in the lysis of target cells by cytolytic T lymphocytes, we probed for the presence of the TNF mRNAs in several quiescent and activated CTL clones. No TNF mRNA could be found in constitutively cytolytic Lyt-2+ clones, and only two out of three clones tested accumulated TNF mRNA after stimulation with phorbol myristate acetate and ionomycin. Of two L3T4+ clones that can be induced to become cytolytic by a combination of antigen and IL-1, only one accumulated TNF-beta mRNA in the process. The PC60 rat X mouse T cell hybrid, which becomes cytolytic in response to a combination of IL-1 and IL-2, also failed to accumulate TNF mRNA after stimulation with these agents. Our results strongly suggest that TNF-alpha or -beta are not necessary agents of the cytolytic activity exhibited by antigen-specific T lymphocytes.This publication has 11 references indexed in Scilit:
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