Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
- 15 February 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (4) , 1229-1233
- https://doi.org/10.1073/pnas.89.4.1229
Abstract
A Xenopus borealis somatic 5S RNA gene was assembled with either the complete octamer of histones, (H2A/H2B/H3/H4)2, or the (H3/H4)2 tetramer of histones that comprises the central protein kernel of the nucleosome. Gel-mobility shifts, DNase I protection, and immunoblotting assays demonstrate that the class III transcription factor IIIA (TFIIIA) readily interacts with 5S DNA associated with the tetramer but that little or no binding is detected when 5S DNA is associated with the full octamer of histones. Thus, the presence of histones H2A and H2B in the 5S nucleosome significantly inhibits the interaction of TFIIIA with its cognate binding site within the 5S RNA gene. We propose that either the depletion of histones H2A and H2B from preexisting nucleosomes or the staged assembly of chromatin after replication in which a tetramer of histones H3/H4 associates with DNA before histones H2A/H2B will facilitate the binding of transcription factors to their cognate DNA sequences.Keywords
This publication has 43 references indexed in Scilit:
- Activation domains of stably bound GAL4 derivatives alleviate repression of promoters by nucleosomesCell, 1991
- Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoterCell, 1990
- Use of selectively trypsinized nucleosome core particles to analyze the role of the histone “tails” in the stabilization of the nucleosomeJournal of Molecular Biology, 1989
- Developmental Regulation of Two 5 S Ribosomal RNA GenesScience, 1988
- Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the Trans-acting factor TFIIIACell, 1987
- Mapping of the sites of protection on a 5 S RNA gene by the Xenopus transcription factor IIIAJournal of Molecular Biology, 1986
- DNA replication in vitro erases a Xenopus 5S RNA gene transcription complexCell, 1986
- Structure of subnucleosomal particles. Tetrameric (H3/H4)2 146 base pair DNA and hexameric (H3/H4)2(H2A/H2B)1 146 base pair DNA complexesBiochemistry, 1985
- Active chromatin of oocytes injected with somatic cell nuclei or cloned DNADevelopmental Biology, 1982
- Assembly of transcriptionally active 5S RNA gene chromatin in vitroCell, 1982