Cooperative interaction of NF‐κB and C/EBP binding sites is necessary for manganese superoxide dismutase gene transcription mediated by lipopolysaccharide and interferon‐γ
- 23 April 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 449 (2-3) , 115-119
- https://doi.org/10.1016/s0014-5793(99)00408-1
Abstract
Expression of the manganese superoxide dismutase (Mn-SOD) is induced by pro-inflammatory cytokines. We investigated the cis-acting elements within a tumor necrosis factor-responsive element (TNFRE) which was identified in the second intron of the murine Mn-SOD gene. Site-directed mutagenesis, reporter plasmid transfection studies and electrophoretic mobility shift assays demonstrated that inducible transcription factors enhanced the transcriptional activity of the Mn-SOD gene through the TNFRE. The cooperation between proteins binding to the newly identified NF-κB and C/EBP sites led to synergistic gene transcription. This report provides the first evidence that cooperation between two distinct cis-acting elements may be required for induction of Mn-SOD gene expression mediated by lipopolysaccharide and interferon-γ.Keywords
This publication has 19 references indexed in Scilit:
- Tumor Necrosis Factor Alpha and Interleukin-1β Regulate the Murine Manganese Superoxide Dismutase Gene Through a Complex Intronic Enhancer Involving C/EBP-β and NF-κBMolecular and Cellular Biology, 1997
- Interaction of NF-κB and NFAT with the Interferon-γ PromoterPublished by Elsevier ,1997
- Irradiation Increases Manganese Superoxide Dismutase mRNA Levels in Human Fibroblasts.Journal of Biological Chemistry, 1995
- Cloning and characterization of the murine manganous superoxide dismutase-encoding geneGene, 1995
- Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide.Proceedings of the National Academy of Sciences, 1993
- C/EBP, NF-kappa B, and c-Ets family members and transcriptional regulation of the cell-specific and inducible macrophage inflammatory protein 1 alpha immediate-early gene.Molecular and Cellular Biology, 1993
- A lymphoid cell-specific nuclear factor containing c-Rel-like proteins preferentially interacts with interleukin-6 kappa B-related motifs whose activities are repressed in lymphoid cells.Molecular and Cellular Biology, 1992
- Constitutive and interleukin-1 (IL-1)-inducible factors interact with the IL-1-responsive element in the IL-6 gene.Molecular and Cellular Biology, 1990
- Nucleotide sequences of cDNAs coding for rat manganese-containing superoxide dismutaseNucleic Acids Research, 1987
- Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiNucleic Acids Research, 1983