Optimizing the sample size and the reduced velocity to achieve maximum production rates of enantiomers
- 1 November 1992
- journal article
- Published by Wiley in Biotechnology Progress
- Vol. 8 (6) , 533-539
- https://doi.org/10.1021/bp00018a010
Abstract
Optimum experimental conditions are predicted for the maximum production rate of D‐ or L‐alanine, purified to a 99% purity from a racemic mixture of their N‐benzoyl derivatives by preparative chromatography on ionically immobilized bovine serum albumin. The experimental production rates and recovery yields measured under these conditions are in excellent agreement with the calculated values. These results validate the approach used for optimization of preparative liquid chromatography.Keywords
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