RADIORECEPTOR ASSAY FOR SERUM-INSULIN
- 1 January 1977
- journal article
- research article
- Vol. 90 (4) , 686-699
Abstract
A method is described for the measurement of insulin in serum by RRA [radioreceptor assay] with normal human s[solubilized]RRA. The sensitivity of the assay is similar to that of a commonly used immunoassay. There is no detectable interference form somatomedin or NSILA [nonsuppressible insulin-like activity], whereas proinsulin has only about 5% of the activity of insulin in he assay. There is a high degree of correlation between the results of immunoassay and the RRA over the physiological range of serum insulin. The greater abiliy to differentiate between insulin and proinsulin, together with greater ability of receptor binding to exclude altered (biologicaly inactive) insulin, present advantages of this assay over the RIA [radioimmunoassay], which may be significant in abnormal situations.This publication has 5 references indexed in Scilit:
- Studies of Insulin, Growth Hormone and Prolactin Binding: Tissue Distribution, Species Variation and CharacterizationEndocrinology, 1974
- Insulin Receptors in Human and Animal Placental TissueDiabetes, 1974
- Coated Charcoal Immunoassay of InsulinJournal of Clinical Endocrinology & Metabolism, 1965
- IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MANJournal of Clinical Investigation, 1960
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951