IMMUNOASSAY OF HUMAN TSH USING DRIED BLOOD SAMPLES1
- 1 May 1976
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 42 (5) , 987-990
- https://doi.org/10.1210/jcem-42-5-987
Abstract
A sensitive, semi-quantitative radioimmunoassay method to screen for elevations of TSH concentration in blood is described. The method requires two 0.32 cm dots of dried blood-impregnated filter paper (equivalent to 3 yl plasma) and a 3-day incubation. Separation of bound and free is obtained using polyethylene glycol. The method can recognize TSH concentrations as low as 22 μU/ml using a highly sensitive antiserum developed by one of us (AFP). TSH in dried cord and newborn blood from 4 infants with congenital hypothyroidism was clearly higher than in normal infants. The method is suitable for use in a newborn screening program to confirm the suspicion of primary hypothyroidism in specimens with low T4 concentrations.Keywords
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