Cross-reaction of FMRFamide with a neuropeptide Y (NPY) antiserum: histological and radioimmunochemical evidence

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Abstract
The presence of neuropeptide Y (NPY) and molluscan cardioexcitatory neuropeptide (FMRFamide) immunoreactivities in blood vessels of rat kidney was demonstrated by indirect peroxidase-anti-peroxidase techniques. However, these immunoreactivities disappeared in rats pretreated with 6-hydroxydopamine (6-OHDA). Radioimmunoassay indicates that FMRFamide possessed the ability to react with NPY-antiserum. The findings suggest that the presence of FMRFamide-immunoreactivity in the rat kidney represents a cross-reactivity of NPY with the FMRFamide-antiserum. Thus, we suggest that only NPY, but not FMRFamide, is located around the vasculatures of the rat kidney.