CULTURED-CELL LINES FOR RESEARCH ON PULMONARY PHYSIOLOGY AVAILABLE THROUGH THE AMERICAN-TYPE-CULTURE-COLLECTION

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Abstract
Procedures are described by which cell lines of potential use for studies on pulmonary physiology were developed or otherwise obtained, characterized and banked for distribution to the scientific community. There were 77 cell lines submitted under this program. Of these, 48 exhibited adequate doubling potential and were of sufficient interest and purity to permit accessioning and banking for distribution. An additional 12 lung cell lines were added earlier during development of the Cell Repository. In all, 4 presumptive type II alveolar lines, 2 non-specified epithelial-like lines, 2 endothelial lines, 1 mesothelial line and 51 fibroblast-like lines are available. Eighteen species are represented. An additional 19 lines were retained as token holdings because they exhibited insufficient doubling potential or represented duplicate samples of differing passage levels. Ten lines were rejected because of poor viability, presence of contaminant microorganisms or inappropriate species identification by the donor. The lines will be retained at the ATCC [American Type Culture Collection] for distribution on request to the scientific community at large. As newly developed strains are identified and made available, these may also be added to the existing collection. Appropriate cooperation from the scientific community and support from governmental funding agencies are required and acknowledged.