The Use of Displacement Chromatography to Alter Retention and Enantioselectivity on a Human Serum Albumin-Based HPLC Chiral Stationary Phase: A Mini-Review

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Abstract
The chromatographic properties of protein-based HPLC chiral stationary phases (CSPs) have been manipulated using a variety of neutral and charged mobile phase modifiers. These compounds have usually been alkyl alcohols and amines, acetonitrile and octanoic acid. This mini-review discusses another approach to the control of chromatographic retention (k′) and enantioselectivity (α) on a human serum albumin based-CSP, HSA-CSP, which utilizes the binding characteristics of the protein. The addition to the mobile phase of compounds known to bind to HSA such as warfarin, ibuprofen and tryptophan altered k′ and α for a variety of solutes. In some instances, direct competition reduced these variables, but the opposite result was also obtained due to allosteric interactions. These interactions, their chromatographic applications and pharmacological implications are examined in this presentation.

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