Stoichiometric binding of 2′(or 3′)-O-(2,4,6-trinitrophenyl)adenosine 5′-triphosphate to bovine heart cytochromecoxidase
- 3 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 307 (3) , 294-296
- https://doi.org/10.1016/0014-5793(92)80698-g
Abstract
The binding of 2′(or 3′)-O-(2,4,6-trinitrophenyl)adenosine 5′-triphosphate (TNP-ATP) to isolated bovine heart cytochrome c oxidase (COX) was studied by following its specific spectral change at 510 nm. The quantitative titration revealed two binding sites for TNP-ATP per monomer COX with a K d of 1.6 μM.Keywords
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