Immunological detection of prohormone convertases in two different proglucagon processing cell lines
- 9 May 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 344 (1) , 65-68
- https://doi.org/10.1016/0014-5793(94)00371-8
Abstract
The distribution of the prohormone convertases, PCI/3, PC2 and PC5/6, was determined by immunoblotting in two cell lines. In αTC1–6 cells, the proglucagon processing occured according to the pancreatic A‐cell type. In STC‐1 cells, proglucagon was processed in a manner reminiscent of the intestinal L‐cell type. PC1/3 was undetectable in both proglucagon processing cell lines whereas PC2 displayed a strong immunostaining in the αTC1–6 cells and was barely detectable in the STC‐1 cells. PC5/6 was detected as a 70 kDa protein in both cell lines. These results suggest a possible role of PC2 in the processing of proglucagon into glucagon in the A‐cells, whereas in L‐cells it would require still undetermined endoproteases.Keywords
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