Method for the Isolation and Liquid Chromatographic Determination of Furazolidone in Chicken Muscle Tissue

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Abstract
A method for the isolation and quantitation of furazolidone as a residue in poultry muscle tissue is presented. Blank control and furazolidone fortified chicken muscle (7.8–250 ng/g tissue) (0.5g) were blended with 2g of octadecylsilyl derivatized silica (C18). The C18/chicken muscle matrix blend was used to prepare a column that was subsequently washed with 8mL of hexane to remove lipids. The furazolidone was then eluted with 8mL of dichloromethane (DCM). The DCM eluate contained furazolidone analyte that was free from interfering compounds when examined by HPLC utilizing UV detection (365nm, photodiode array). The extracted standard curves (linear regression analysis correlation r=0.9995 /pm 0.0002, n=5), average percentage recovery (99.8/pm 4.42%, the Major Metabolite of Δ-9-Tetrahydrocannabinol in Urine., J. Anal. Toxicol., 2, 6–9 (1985).