Spectrophotometric Determination of Ferric, Ferrous, and Total Iron in Drugs by Reaction with α,α′-Dipyridyl

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Abstract
The drug sample is dissolved in 0.2N HCl to yield a final concentration of 30 μg Fe/ml, an aliquot is reacted with α,α′-dipyridyl, and the pH is adjusted with an acetate buffer. For Fe(II), the color intensity is measured at 523 nm. For total iron, ascorbic acid is added to a separate aliquot of the sample solution, reducing all the iron present to Fe(II), which is then reacted with α,α′-dipyridyl, diluted to volume, and read at 523 nm. The Fe(III) content is determined by subtracting Fe(II) from total iron. This method is applicable to iron-containing drugs and drug dosage forms.

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