Towards understanding the catalytic core structure of the spliceosome
- 1 June 2005
- journal article
- review article
- Published by Portland Press Ltd. in Biochemical Society Transactions
- Vol. 33 (3) , 447-449
- https://doi.org/10.1042/bst0330447
Abstract
The spliceosome catalyses the splicing of nuclear pre-mRNA (precursor mRNA) in eukaryotes. Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The spliceosome is a complex assembly of five RNAs (U1, U2, U4, U5 and U6) and many dozens of associated proteins. Although a high-resolution structure of the spliceosome is not yet available, inroads have been made towards understanding its structure and function. There is growing evidence suggesting that U2 and U6 RNAs, of the five, may contribute to the catalysis of pre-mRNA splicing. In this review, recent progress towards understanding the structure and function of U2 and U6 RNAs is summarized.Keywords
This publication has 28 references indexed in Scilit:
- U2–U6 RNA folding reveals a group II intron-like domain and a four-helix junctionNature Structural & Molecular Biology, 2004
- Roles of the U5 snRNP in spliceosome dynamics and catalysisBiochemical Society Transactions, 2004
- New tertiary constraints between the RNA components of active yeast spliceosomes: A photo-crosslinking studyRNA, 2004
- Multiple functions for the invariant AGC triad of U6 snRNARNA, 2004
- Single-molecule studies of DNA and RNA four-way junctionsBiochemical Society Transactions, 2004
- Structure of the U6 RNA intramolecular stem–loop harboring an SP-phosphorothioate modificationRNA, 2003
- Metal binding and base ionization in the U6 RNA intramolecular stem-loop structureNature Structural & Molecular Biology, 2002
- Spliceosomal snRNAs: Mg2+-Dependent Chemistry at the Catalytic Core?Cell, 2002
- Randomization-selection analysis of snRNAs in vivo: evidence for a tertiary interaction in the spliceosome.Genes & Development, 1994
- Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicingNature, 1993