The effect of milk and lactobacillus feeding on human intestinal bacterial enzyme activity

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Abstract
Twenty-one subjects were recruited for a study designed to investigate the effect of oral supplements of Lactobacillus acidophilus on fecal bacterial enzyme activity. Three bacterial enzymes were assayed: β-glucuronidase, nitroreductase, and azoreductase. These fecal enzymes can catalyze procarcinogens conversion to a proximal carcinogen. The sequence of feeding studies and fecal enzyme assays was the same for all subjects:4 wk of a control period; 4 wk of plain milk feeding; 4 wk of control, without any dietary supplements; 4 wk of milk containing 2 × 106 per ml viable L acidophilus; and 4 wk of control, without any supplements. The concentration of viable lactobacilli simulates that found in acidophilus supplemented milk. Reductions of 2- to 4-fold in the activities of the three fecal enzymes were observed only during the period of lactobacilli feeding. These changes were noted in all subjects and were highly significant (p < 0.02 to 0.001). During the final control period, after lactobacilli feeding, fecal enzyme levels returned to normal after 4 wk.