Activation of Chemiluminescent Oxidations Catalyzed by Peroxidase and the Differentiation of Peroxidase Agents.

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Abstract
The enzymic oxidation of pyrogallol by peroxide in the presence of peroxidases is accompanied by chemiluminescence. The luminescent reaction may under certain conditions be considerably activated by addition of certain non-luminescent hydrogen donors, such as o-phenyl-enediamine. This activation is evidently due to the coupled oxidation of pyrogallol or a reaction product thereof by the primarily formed reaction products derived from the "activator". The presence of non-luminescent donor is advantageous when determining low enzyme activities; the effect observed by simultaneous oxidation of luminescent and non-luminescent donor on the luminescent oxidation of pyrogallol varies with the peroxidatic agent used, and the system may be utilized for differentiating between HRP, hemoglobin and myeloperoxidase.

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