Immunodetection and photostability of NADPH-protochlorophyllide oxidoreductase in Pinus pinea L.
- 1 January 1990
- journal article
- research article
- Published by Springer Nature in Photosynthesis Research
- Vol. 23 (1) , 89-94
- https://doi.org/10.1007/bf00030067
Abstract
Antibody against the light-dependent NADPH-protochlorophyllide oxidoreductase of oat was used to detect a protein of the same molecular weight in cotyledons of 40-day-old dark-grown seedlings of Pinus pinea L. Exposure of the seedlings to light resulted in a rapid decrease in protochlorophyllide content without the concomitant decrease in 38 kDa protein which is observed on transfer of dark-grown angiosperm seedlings to light. The stability of the light-dependent NADPH-protochlorophyllide oxidoreductase in pine in the absence of accumulated substrate is consistent with either (1) a different mechanism of regulation of chlorophyll synthesis in gymnosperms or (2) a higher proportion of stable extra-plastidic protein reacting with the antibody to the light-dependent NADPH-protochlorophyllide oxidoreductase than is the case in angiosperms.Keywords
This publication has 24 references indexed in Scilit:
- A comparison of prolamellar bodies from wheat, Scots pine and Jeffrey pine. Pigment spectra and properties of protochlorophyllide oxidoreductasePhysiologia Plantarum, 1987
- Chlorophyll synthesis in green leaves and isolated chloroplasts of barley (Hordeum vulgare)Physiologia Plantarum, 1987
- Characterization of prolamellar bodies, from dark‐grown seedlings of Scots pine, containing light‐ and NADPH‐dependent protochlorophyllide oxidoreductasePhysiologia Plantarum, 1986
- Localization of NADPH‐protochlorophyllide oxidoreductase in dark‐grown wheat (Triticum aestivum) by immuno‐electron microscopy before and after transformation of the prolamellar bodiesPhysiologia Plantarum, 1986
- Fast and efficient method for detection and estimation of proteinsBiochemical and Biophysical Research Communications, 1985
- Light‐independent accumulation of chlorophyll a and b and protochlorophyllide in green barley (Hordeum vulgare)Physiologia Plantarum, 1985
- Light-Induced Breakdown of NADPH-Protochlorophyllide Oxidoreductase In VitroPlant Physiology, 1983
- The Protochlorophyllide Holochrome of Barley (Hordeum vulgare L.)European Journal of Biochemistry, 1981
- Plastid differentiation and chlorophyll synthesis in cotyledons of black pine seedlings grown in the darkProtoplasma, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970