The E. coli K-12 chromosome flanked by two IS10 sequences transposes
- 1 October 1984
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 197 (1) , 62-66
- https://doi.org/10.1007/bf00327923
Abstract
Transposon are commonly found among prokaryotes and usually range up to 20 kilobases. In this study, we were interested to determine whether a larger DNA segment could transpose. We observed that the E. coli K-12 chromosome, 4,000 kilobases in size, when flanked by two IS10 sequences, could transpose to pACYC177 at a frequency of 10-8 per cell per generation. We suggest that this transposition event occurs independently of the size and without duplication of the entire DNA sequence flanked by the IS10 elements.This publication has 36 references indexed in Scilit:
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