Evidence of septic system failure determined by a bacterial biochemical fingerprinting method
- 1 April 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 98 (4) , 910-920
- https://doi.org/10.1111/j.1365-2672.2004.02522.x
Abstract
Aims: To provide evidence of septic system failure by comparing two faecal indicator bacteria, enterococci and Escherichia coli, from defective septic tanks and adjacent creeks. Methods and Results: A biochemical fingerprinting method was used to type and compare enterococci and E. coli strains from 39 septic tanks with creek water samples. Phenotypic diversity of enterococci (0·5 ± 0·3) and E. coli (0·5 ± 0·3) in septic tanks were significantly lower than those found in water samples (0·8 ± 0·1, P < 0·0001 for enterococci and 0·9 ± 0·1, P < 0·0001 for E. coli). Among 1072 enterococci isolates tested from septic tanks, 203 biochemical phenotypes (BPTs) were found of which 98 BPTs from 33 septic tanks were identical to several water samples. Similarly, among 621 E. coli isolates tested from septic tanks, 159 BPTs were found of which 53 BPTs from 26 septic tanks were also identical to water samples. The number of the latter bacteria was significantly (P = 0·01) higher in water samples collected from downstream compared with that of upstream in the study area. A high similarity between the populations of both indicator bacteria was also found between defective septic tanks and downstream water samples further indicating the contamination of both creeks by defective septic systems. Conclusions: Biochemical fingerprinting of faecal indicator bacteria is a useful and rapid method to provide direct evidence for septic system failure. Combination of both faecal indicator bacteria (enterococci and E. coli) provides a better judgement of the performance of a septic system. Significance and Impact of the Study: This study is the first to provide direct evidence of septic system failure by identifying the presence of specific bacterial types in septic tanks and surface waters. Based on our findings, we suggest that the performance evaluation of a septic system should be accompanied by direct analysis of faecal indicator bacteria.Keywords
This publication has 27 references indexed in Scilit:
- High Prevalence of Vancomycin-Resistant Enterococci in Swedish SewageApplied and Environmental Microbiology, 2002
- Identification of Fecal Escherichia coli from Humans and Animals by RibotypingApplied and Environmental Microbiology, 2001
- Marine Recreation and Public Health Microbiology: Quest for the Ideal IndicatorBioScience, 2001
- Classification of Antibiotic Resistance Patterns of Indicator Bacteria by Discriminant Analysis: Use in Predicting the Source of Fecal Contamination in Subtropical WatersApplied and Environmental Microbiology, 2000
- Use of Repetitive DNA Sequences and the PCR To Differentiate Escherichia coli Isolates from Human and Animal SourcesApplied and Environmental Microbiology, 2000
- Biochemical fingerprinting of coliform bacterial populations - comparisons between polluted river water and factory effluentsWater Science & Technology, 1997
- Evaluation of escherichia coli as the main indicator of faecal pollutionWater Science & Technology, 1997
- Microbiological Quality of Source Waters for Water SupplyPublished by Springer Nature ,1990
- The Soil as a Renovating Medium — Clogging of Infiltrative SurfacesPublished by Springer Nature ,1982
- Site Evaluation and Design of Seepage FieldsJournal of the Environmental Engineering Division, 1974