DEFECTIVE REMOVAL OF DNA CROSS-LINKS IN A REPAIR-DEFICIENT MUTANT OF CHINESE-HAMSTER CELLS

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (8) , 3106-3110
Abstract
To further understand the relationships between DNA damage, DNA repair and cellular end points such as survival and mutation, the repair capacity of a DNA repair-deficient mutant (strain UV-20) of Chinese hamster ovary cells was characterized in response to DNA cross-linking agents. This mutant, previously shown to be hypersensitive to killing by both UV light and the cross-linking agent mitomycin C, was also found to be extremely sensitive to cis-diamminedichloroplatinum, another DNA cross-linking agent. The efficiency of DNA cross-link removal after treatment with mitomycin C or cis-diamminedichloroplatinum was measured using the technique of alkaline elution and compared in wild-type Chinese hamster ovary cells and strain UV-20. Wild-type cells removed 80 or 95% of the cross-links within 24 h after treatment with cis-diamminedichloroplatinum or mitomycin C, respectively. UV-20 cells, which were equally as susceptible to cross-link damage as were wild-type cells, removed only a small proportion of the cross-links made by either agent. The importance of DNA repair processes in modulating the cytotoxic effects of chemicals that produce DNA cross-link damage is emphasized. Cross-link repair in Chinese hamster ovary cells may be controlled by a pathway that also repairs damage from UV radiation.

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