Coupling of Mitosis to the Completion of S Phase Through Cdc34-Mediated Degradation of Wee1
- 4 December 1998
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 282 (5395) , 1886-1889
- https://doi.org/10.1126/science.282.5395.1886
Abstract
The dependence of mitosis on the completion of the period of DNA replication in the cell cycle [synthesis (S) phase] ensures that chromosome segregation occurs only after the genome has been fully duplicated. A key negative regulator of mitosis, the protein kinase Wee1, was degraded in a Cdc34-dependent fashion in Xenopusegg extracts. This proteolysis event was required for a timely entrance into mitosis and was inhibited when DNA replication was blocked. Therefore, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee1 during S phase.Keywords
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