The mode of actions of glyceraldehyde‐3‐phosphate dehydrogenase identified as an immunoglobulin production stimulating factor
- 10 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (1) , 92-96
- https://doi.org/10.1016/0014-5793(95)00607-b
Abstract
Immunoglobulin production stimulating factor-II alpha, which enhances immunoglobulin production of human and mouse hybridomas was purified from cell lysate of human Burkitt's lymphoma, Namalwa cells, and identified as glyceraldehyde-3-phosphate dehydrogenase. The enhancement of immunoglobulin production with this enzyme was not linked with its enzymatic activity. The enzyme enhanced immunoglobulin productivity of transcription-suppressed hybridomas, but did not enhance that of translation-suppressed hybridomas. From these results, it is suggested that this enzyme takes part in the post-translational control or the enhancement of translation activity to stimulate immunoglobulin production of hybridomas.Keywords
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