Use of lactate dehydrogenase release to assess changes in culture viability
- 1 May 1990
- journal article
- conference paper
- Published by Springer Nature in Cytotechnology
- Vol. 3 (3) , 301-307
- https://doi.org/10.1007/bf00365494
Abstract
This study reports the use of lactate dehydrogenase release to monitor changes in culture viability in flask culture and fixed bed, porosphere bioreactor systems. Lactate dehydrogenase release shows good agreement with increase in non-viable cell numbers and decline in glucose utilisation in flask cultures. Studies with the immobilised system show that lactate dehydrogenase release can detect loss of viability which is not always indicated by a decrease in glucose utilisation. The data show that culture viability in a repeated-feed-and-harvest system is influenced markedly by both a) the medium change regime itself and b) the use of an immobilised bioreactor compared to a flask system for the same medium change regime.Keywords
This publication has 7 references indexed in Scilit:
- Viability measurements in mammalian cell systemsAnalytical Biochemistry, 1989
- Fixed bed porous glass sphere (porosphere) bioreactors for animal cellsCytotechnology, 1988
- Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentrationJournal of Biotechnology, 1988
- Factors affecting cell growth and monoclonal antibody production in stirred reactorsJournal of Immunological Methods, 1986
- Variation in the susceptibility of BHK populations and cloned cell lines to three strains of foot-and-mouth disease virusArchiv für die gesamte Virusforschung, 1980
- Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblastsJournal of Cellular Physiology, 1978
- Energy metabolism in respiration‐deficient and wild type chinese hamster fibroblasts in cultureJournal of Cellular Physiology, 1976