Hormone-stimulated lipolysis in cardiac myocytes

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Abstract
Type L hormone-sensitive lipase (HSL) activity was increased approximately 35% above control in [rat] cardiac myocytes incubated for 15 min with 5 nM adrenaline [epinephrine]. Concomitantly, adrenaline-stimulated myocytes had a lower triacylglycerol content, released more nonesterified fatty acid and had a higher intracellular concentration of cAMP than did myocytes incubated without hormone. The lipase activity measured in adrenaline-stimulated and nonstimulated myocytes was stable in acetone/diethyl ether, stimulated by serum and inhibited by NaCl. These properties are consistent with the type L designation of this HSL. The finding that type L HSL is stimulated by adrenaline indicates that the enzyme that is being activated is found in the cell and not associated with an extracellular compartment of the myocardium.