ESTROGEN AND ANTIESTROGEN BINDING TO DIFFERENT FORMS OF THE MOLYBDATE-STABILIZED ESTROGEN RECEPTOR

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Abstract
We report that the calf uterine estrogen receptor, prepared in a Tris-raolybdate buffer, bound by 10 nM [3H]estradiol and eluted by a KCl gradient from DEAE-cellulose columns, yielded only one very sharp receptor peak. Estrogen receptor prepared in phosphate buffer with molybdate and eluted with KCl also yielded only one sharp peak on DEAE-cellulose. However, if DEAE-Sephadex (with phosphate buffer plus molybdate) was used, the [3H]estradiol-receptor complex eluted with two sharp peaks at approximately 0.21 and 0.25 M KCl (Peaks I and II, respectively). But the high-affinity antiestrogen, [3H]H1285, bound to estrogen receptor, eluted only as Peak I and not as Peak II. Both the [3H]estradiol and [3H]H1285 binding peaks were saturable since they could be eliminated with 200-fold excess estradiol. Therefore, ion exchangechromatography using different resins and/or buffers may be useful for determining physicochemicai differences in estrogen versus antiestrogen receptor complexes.

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