The Separation of a Protective Antigen from a Toxin-Producing Strain of Hemophilus Pertussis

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Abstract
Summary: The serological characteristics of a toxin producing strain of H. pertussis, # 29, are compared with a typical phase I strain, # 18-323. A water extract of strain # 29 organisms, heated at 56 C for 45 minutes, contains a protective antigen against a typical phase I strain, # 18-323, the challenge organism in the mouse protection test. The protective antigen can be partially separated from the heated, non-toxic, water extract by fractionation with methanol-water mixtures under controlled conditions of pH, ionic strength and temperature.