Intracellular Ca2+ imaging for micropatterned cardiac myocytes
- 14 January 2003
- journal article
- other
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 81 (6) , 748-751
- https://doi.org/10.1002/bit.10521
Abstract
The patterning of cardiac myocytes on a micron scale (∼5 μm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca2+ transition over the pattern, and thus simultaneously contracted. The dynamic change of the Ca2+ concentration within the patterned tissue was analyzed quantitatively during successive contraction and relaxation using a Nipkow-type high-speed confocal microscope. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 748–751, 2003.Keywords
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