Membrane Enzyme Development in Nerve Ending Mitochondria during Neonatal Hypothyroidism

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Abstract
The effect of propylthiouracil-induced neonatal hypothyroidism on the development of [rat] synaptosome fraction mitochondrial membrane enzymes was determined. Total and specific activities of 2 mitochondrial inner membrane (cytochrome c oxidase and succinate cytochrome c reductase) and 2 outer membrane enzymes (monoamine oxidase and rotenone-insensitive NADH cytochrome c reductase) were determined. The inner mitochondrial membrane enzymes developed in synchrony in euthyroid animals, but a decrease in fractional and specific activities of both inner membrane enzymes occurred in hypothyroidism. The outer membrane enzymes developed asynchronously; monoamine oxidase revealed a 9-fold increase while NADH cytochrome c reductase increased 4-fold. Hypothyroidism caused no change in total fractional activity or Km and an increased specific activity of outer membrane enzymes.