EFFECT OF AET TREATMENT OF SHEEP ERYTHROCYTES ON THE ROSETTE TEST IN VARIOUS DISEASES

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Abstract
Patients (180) with neurological or infectious diseases and 90 normal controls were examined for blood T lymphocyte percentage by the standard method using rosette formation with untreated sheep erythrocytes (E) and a technique using 2-aminoethylisothiuronium bromide hydrobromide (AET)-treated E, with fetal calf serum (FCS) in the medium. Patients with acute meningitis had a decrease and those with infectious mononucleosis had an increase in T lymphocyte percentage as measured by both assays. Patients with acute MS [multiple sclerosis] and with [malignant] cerebral tumors had a decrease in T cell percentage by the E but not by the EAET technique. The T lymphocyte percentages of the other 4 patient groups did not differ significantly from those of the controls, regardless of the technique used. In certain diseases, the use or not of AET-treated indicator cells may influence the results of the rosette test.