Effect of transforming growth factor beta and basic fibroblast growth factor on steroid-impaired healing intestinal wounds

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Abstract
A longitudinal intestinal wound model in the pig was used to assess the effect of parenteral steroids (betamethasone 12 mg 50 kg−1 intramuscularly twice daily) on breaking load. Steroid treatment significantly decreased the breaking load of wounds in the ileum and colon in comparison with wounds from saline‐treated animals. In a further group of animals receiving steroids, paired longitudinal wounds were constructed. One wound of a pair was treated with a local application of transforming growth factor beta (TGF‐β) (5 μg per wound) or basic fibroblast growth factor (5 μg per wound) in a collagen suspension. The other wound was treated with a collagen suspension alone. Ileal wounds treated with TGF‐β were significantly stronger than collagen‐treated controls at 7 days. The steroid‐induced impairment of breaking load in intestinal wounds is partially reversed by a local application of TGF‐β in a collagen suspension at the time of surgery.