On the mechanism of spontaneous transfer of lipids from isolated microsomal to mitochondrial membranes

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Abstract
Spontaneous (protein independent) transfer of endogenously biosynthesized radioactive lipids (phosphatidic acid, phosphatidylcholine and diglycerides) from isolated guinea pig liver microsomal to unlabeled mitochondrial membranes was studied as a function of addition of ATP, duration of incubation, and protein concentration of microsomal and mitochondrial membranes. Transfer of phosphatidic acid, phosphatidylcholine and diglycerides was not absolutely dependent on addition of ATP, although its presence enhanced the transfer of phosphatidylcholine. The highest amount of transferred biosynthesized phosphatidic acid, phosphatidylcholine, and diglycerides was observed after a relatively short incubation and amounted to around 1/3 of all biosynthesized microsomal lipids, but decreased with increasing incubation time. With increasing concentrations of proteins of mitochondria, a significant decrease of transfer of radioactive lipids from microsomal membranes was established. The same tendency, although to a lesser degree, was established with increasing concentrations of microsomal proteins.

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