Feedback Control of theArabidopsisHypersensitive Response
Open Access
- 1 April 2004
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 17 (4) , 357-365
- https://doi.org/10.1094/mpmi.2004.17.4.357
Abstract
The plant hypersensitive response (HR) to avirulent bacterial pathogens results from programmed cell death of plant cells in the infected region. Ion leakage and changes in signaling components associated with HR progression were measured. These studies compared Arabidopsis mutants affecting feedback loops with wild-type plants, with timepoints taken hourly. In response to Pseudomonas syringae pv. tomato DC3000 x avrB, npr1-2 mutant plants showed increased ion leakage relative to wild-type plants. Hydrogen peroxide accumulation was similar to that in wild type, but salicylic acid accumulation was reduced at some timepoints. With DC3000 x avrRpt2, similar trends were seen. In response to DC3000 x avrB, ndr1-1 mutant plants showed more ion leakage than wild-type or npr1-2 plants. Hydrogen peroxide accumulation was delayed by approximately 1 h and reached half the level seen with wild-type plants. Salicylic acid accumulation was similar to npr1-2 mutant plants. With DC3000 x avrRpt2, ndr1-1 mutant plants showed no ion leakage, no hydrogen peroxide accumulation, and minimal salicylic acid accumulation. Results with a ndr1-1 and npr1-2 double mutant were similar to ndr1-1. A model consistent with these data is presented, in which one positive and two negative regulatory circuits control HR progression. Understanding this circuitry will facilitate HR manipulation for enhanced disease resistance.Keywords
This publication has 57 references indexed in Scilit:
- A Dynamic Mathematical Model To Clarify Signaling Circuitry Underlying Programmed Cell Death Control in Arabidopsis Disease ResistanceBiotechnology Progress, 2004
- Quantitative Nature of Arabidopsis Responses during Compatible and Incompatible Interactions with the Bacterial PathogenPseudomonas syringae [W]Plant Cell, 2003
- Arabidopsis RIN4 Is a Target of the Type III Virulence Effector AvrRpt2 and Modulates RPS2-Mediated ResistanceCell, 2003
- Preexisting Systemic Acquired Resistance Suppresses Hypersensitive Response-Associated Cell Death in Arabidopsishrl1 MutantPlant Physiology, 2002
- RIN4 Interacts with Pseudomonas syringae Type III Effector Molecules and Is Required for RPM1-Mediated Resistance in ArabidopsisCell, 2002
- Programmed cell death, mitochondria and the plant hypersensitive responseNature, 2001
- Involvement of reactive oxygen species in the response of resistant (hypersensitive) or susceptible cowpeas to the cowpea rust fungusNew Phytologist, 1998
- NDR1 , a Pathogen-Induced Component Required for Arabidopsis Disease ResistanceScience, 1997
- Characterization of aSalicylicAcid-Insensitive Mutant (sai1) ofArabidopsis thaliana, Identified in a Selective Screen Utilizing the SA-Inducible Expression of thetms2GeneMolecular Plant-Microbe Interactions®, 1997
- Concurrent Loss in Tn5Mutants ofPseudomonas syringaepv.syringaeof the Ability to Induce the Hypersensitive Response and Host Plasma Membrane K+/H+Exchange in TobaccoPhytopathology®, 1987