Entirely Automated Quantification of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Plasma by Using the Ultrasensitive COBAS AMPLICOR HIV-1 Monitor Test and RNA Purification on the MagNA Pure LC Instrument
Open Access
- 1 March 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (3) , 1248-1251
- https://doi.org/10.1128/jcm.41.3.1248-1251.2003
Abstract
The ultrasensitive COBAS AMPLICOR HIV-1 Monitor test was complemented with automated RNA purification on the MagNA Pure LC instrument. This enabled entirely automated ultrasensitive assessment of viral loads in human immunodeficiency virus type 1 (HIV-1)-infected individuals. The detection limit of the fully automated assay and the viral load measurements in 80 clinical samples were found to be in good agreement with those of the conventional ultrasensitive COBAS AMPLICOR HIV-1 Monitor test. The fully automated assay showed markedly reduced hands-on time and was found to be suitable for the routine assessment of HIV-1 viral loads in a clinical diagnostic laboratory.Keywords
This publication has 22 references indexed in Scilit:
- Antiretroviral Treatment for Adult HIV Infection in 2002JAMA, 2002
- Highly Active Antiretroviral Therapy Decreases Mortality and Morbidity in Patients with Advanced HIV DiseaseAnnals of Internal Medicine, 2001
- Fully Automated Nucleic Acid Extraction: MagNA Pure LCClinical Chemistry, 2001
- Detection of Herpes Simplex Virus DNA in Genital and Dermal Specimens by LightCycler PCR after Extraction using the IsoQuick, MagNA Pure, and BioRobot 9604 MethodsJournal of Clinical Microbiology, 2001
- Prevalence of Human Immunodeficiency Virus Type 1 (HIV-1) Non-B Subtypes in Foreigners Living in Madrid, Spain, and Comparison of the Performances of the AMPLICOR HIV-1 MONITOR Version 1.0 and the New Automated Version 1.5Journal of Clinical Microbiology, 2001
- Automation of laboratory testing for infectious diseases using the polymerase chain reaction — our past, our present, our futureJournal of Clinical Virology, 2001
- Predictive value of the ultrasensitive HIV viral load assay in clinical practiceJournal of Medical Virology, 2000
- Decrease of HIV-1 RNA levels in lymphoid tissue and peripheral blood during treatment with ritonavir, lamivudine and zidovudineAIDS, 1998
- Treatment with Indinavir, Zidovudine, and Lamivudine in Adults with Human Immunodeficiency Virus Infection and Prior Antiretroviral TherapyNew England Journal of Medicine, 1997
- HIV viral load markers in clinical practiceNature Medicine, 1996