Degradation in vivo of the C3 protein of guinea-pig complement by a pathogenic strain of Bacteroides gingivalis

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Abstract
The pathogenicity of 5 black-pigmented strains of Bacteroides was tested in s.c. implanted Teflon cages in guinea pigs. The tissue reaction around the cages was registered and the contents of the fluid of the cages were analyzed. Two strains of B. intermedius produced a localized abscess around the cages; 1 strain (381) of B. gingivalis and an asaccharolytic strain (BN11a-f) different from B. gingivalis did not induce any signs of abscess formation. One strain (W83) of B. gingivalis caused extensive purulent breakdown of the tissues. When the inoculum of strain W83 contained > 109 cells, the animals were killed. Strain W83 was the only strain that increased in number in the cage. The fluid of cages inoculated with strain W83 was also remarkably different from the fluid of cages inoculated with the other strains. The fluid had a high proteolytic activity. No C3 protein of complement and only traces of Ig were detected in the fluid. Strain W83 and strain 381 had a high proteolytic activity against whole guinea pig serum; when these 2 strains were incubated with guinea pig serum for 24 h, almost all serum proteins, including the C3 protein, were degraded. These 2 strains might thus have similar capacity in perturbing the host defense when inoculated into the tissue cages. The actual difference in pathogenicity between the strains might be explained by a recent finding that the pathogenic strain w83, but not strain 381, requires complement in activating polymorphonuclear leukocytes. The degradation of the C3 protein by the pathogenic strain W83 of B. gingivalis thus may be the crucial event in its perturbation of the host defense. A degradation of the C3 protein by strain 381 would be of no help in eluding the host defense, since this strain activates polymorphonuclear leukocytes in the absence of complement.