Plant Breeding of Cannabis. Determination of Cannabinoids by High-Pressure Liquid Chromatography

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Abstract
Cannabinoids (CN) of CBDA stock, Tochishi No. 1 stock and crossed stock were determined on a high-pressure liquid chromatograph equipped with a LiChrosorb RP-18 column (25 cm .times. 4 mm) by elution with a mixture of methanol and 0.02 N sulphuric acid (4: 1). The CBDA stock contained cannabidiolic acid (CBDA) and cannabichromenic acid (CBCA). Tochishi No. 1 stock had tetrahydrocannabinolic acid (THCA), CBCA and a small amount of CBDA. F1 was obtained by crossing CBDA stock (female) and Tochishi No. 1 stock (male). All 79 F1 specimens contained CBDA, THCA and CBCA. F1 was designated the median type. F2 was then obtained by crossing F1 and out of 199 F2 specimens, 58, 40 and 101 stocks were designated as CBDA stock type, Tochishi No. 1 stock type and the median type by CN analysis, respectively. F3 was also gained by crossing CBDA stock type of F2 and all 166 F3 specimens were CBDA stock type. F4, which was obtained by crossing F3, was also CBDA stock type. The fibers of F4 were better than those of the original CBDA stock and F4 contained a negligible amount of THCA.

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