A Fluorescence Quenching Method for the Determination of Bromate Ion with 4,5-Dibromophenylfluorone and Cetyltrimethylammonium Bromide

Abstract
A highly sensitive and selective fluorescence quenching method was developed to determine the bromate ion (BrO3 ) with 4,5-dibromophenylfluorone (DB-PF) as fluorogenic reagent. BrO3 reacts with potassium bromide in sulfuric acid solution (0.6M) giving bromine (Br2) which is estimated with fluorescence quenching method using DB-PF as fluorogenic reagent. Bromine reacts with DB-PF to produce a compound, whose maximum excitation wavelength and emission wavelength are 543nm, 560nm respectively. The linear calibration range is 0.05–0.5 μg/25ml. The detection limit is 0.05μg/25ml. The method may be used to determine microamounts of BrO3 in potassium chlorate with satisfactory results. The method offers the advantages of simplicity, rapidity and sensitivity.

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