Effect of maleimide derivatives, sulfhydryl reagents, on stimulation of neutrophil superoxide anion generation with concanavalin A

Publisher Website
Google Scholar
Abstract
Using maleimide derivatives with relatively rare side reactions, their effect on stimulation of O‐ 2 production by guinea‐pig neutrophils with Con A was studied. Showdomycin, a very slowly penetrating agent, did not affect O‐ 2 production whereas NEM, a rapidly penetrating agent, markedly inhibited O‐ 2 production without interference with binding of Con A to cells. Particulate fractions from neutrophils stimulated with Con A showed markedly increased NADPH‐dependent O‐ 2 production compared with fractions from unstimulated cells. Treatment of neutrophils with NEM before exposure to Con A inhibited the enhancement of NADPH‐dependent O‐ 2 production of particulate fractions by Con A. However, particulate fractions from Con A‐stimulated and unstimulated cells hardly exhibited reduced NADPH oxidase activity after direct exposure to NEM. Treatment of neutrophils with NEM after activation by Con A had no effect on NADPH‐dependent O‐ 2 production of particulate fractions. These results indicate that NEM inhibits the activation process of the O‐ 2‐generating enzyme, probably NADPH oxidase with Con A.

This publication has 20 references indexed in Scilit: