Identification and properties of two methyltransferases in conversion of phosphatidylethanolamine to phosphatidylcholine.
- 1 April 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (4) , 1718-1721
- https://doi.org/10.1073/pnas.75.4.1718
Abstract
Two methyltransferases involved in the methylation of phosphatidylethanolamine to form phosphatidylcholine were demonstrated in a microsomal fraction of bovine adrenal medulla. The 1st methyltransferase catalyzes the methylation of phosphatidylethanolamine to form phosphatidyl-N-monomethylethanolamine. This enzyme has an optimum pH of 6.5, a low Km for S-adenosyl-L-methionine (1.4 .mu.M), and an absolute requirement for Mg2+. The 2nd methyltransferase catalyzes the 2 successive methylations of phosphatidyl-N-monomethylethanolamine to phosphatidyl-N,N-dimethylethanolamine and phosphatidylcholine. In contrast to the 1st methyltransferase, it has an optimum pH of 10 and a high Km for S-adenosyl-L-methionine (0.1 mM) and does not require Mg2+.This publication has 11 references indexed in Scilit:
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