Determination of Two Binary Vitamin Mixtures Using Difference and Derivative Spectrophotometry

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Abstract
Simple, sensitive, rapid and accurate spectrophotometric procedures are described for the analysis of binary mixtures of vitamins. The first mixture: pyridoxine hydrochloride (PYRD) and thiamine hydrochloride (THIA): is analyzed using a combination of first- and third- derivative spectrophotometry for the determination of PYRD and THIA, respectively. The method is applied for the analysis of turbid mixtures and adopted for studying the dissolution of tablets. The second mixture; menadione sodium biphosphate (MENA) and ascorbic acid (ASCO); is analyzed using a second derivative and difference spectrophotometric methods for determination of MENA and ASCO, respectively. The validation of the proposed methods is discussed and their application for the analysis of tablets and ampoules are presented.