Indirect Fluorescent Antibody Techniques for Demonstration of Trout Viruses and Corresponding Antibody
Open Access
- 1 June 1974
- journal article
- research article
- Published by Springer Nature in Acta Veterinaria Scandinavica
- Vol. 15 (2) , 198-205
- https://doi.org/10.1186/bf03547481
Abstract
Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT allowed demonstration of trout antibody to the viruses. Antibody, by means of the latter technique, could be demonstrated only in artificially immunized trout.Keywords
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