Protein and phospholipid methylation during chemotaxis in Dictyostelium discoideum and its relationship to calcium movements.
- 30 November 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (12) , 6106-6109
- https://doi.org/10.1073/pnas.76.12.6106
Abstract
Suspensions of cyclic[c]AMP sensitive cells of D. discoideum responded to a cAMP pulse with increased methylation of a protein of MW about 120,000 and increased phospholipid demethylation. Protein methylation reached its peak 15-30 s after cAMP addition. Phospholipid demethylation reached its maximum within 2 min and basal levels were recovered in 3 min. S-Adenosyl-L-methionine is probably the methyl donor. In vitro addition of 0.25 mM and 25 .mu.M S-adenosyl-L-methionine to sonicated D. discoideum cells inhibited ATP-dependent 45Ca2+ uptake by 70 and 25%, respectively. Based on this evidence it is proposed that protein and phospholipid methylation are involved in D. discoideum chemotaxis probably by regulation of intracellular Ca2+ movements.This publication has 22 references indexed in Scilit:
- S‐Adenosylmethionine inhibits Ca2+ uptake into Escherichia coli membrane vesiclesFEBS Letters, 1979
- Increased Ca2+-ATPase activity associated with methylation of phospholipids in human erythrocytesBiochemical and Biophysical Research Communications, 1979
- Bacterial chemotaxis and protein carboxymethylationNature, 1979
- beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling.Proceedings of the National Academy of Sciences, 1979
- Guanylate cyclase activation in response to chemotactic stimulation in Dictyostelium discoideumFEBS Letters, 1978
- 3′:5′-Cyclic AMP-dependent 3′:5′-cyclic GMP accumulation in Dictyostelium discoideumProceedings of the National Academy of Sciences, 1977
- Cyclic GMP in Dictyostelium discoideum Oscillations and pulses in response to folic acid and cyclic AMP signalsFEBS Letters, 1977
- Receptor‐mediated adenylate cyclase activation in Dictyostelium discoideumFEBS Letters, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Cell aggregation in Dictyostelium discoideumDevelopmental Biology, 1961