Removal of Bacteria from Beef Tissue by Spray Washing after Different Times of Exposure to Fecal Material

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Abstract
This study examined the effect of exposure time of beef-fat fascia to an inoculated fecal paste on the efficiency of removal of bacteria by spray washing or rinsing. Brisket fat (obtained 8 CFU/g) of streptomycin-resistant Escherichia coli ATTC 11370. Triplicate samples were spray washed with water (35°C, 20.7 bar) and then rinsed with solutions of 2% acetic acid, 5% hydrogen peroxide, or 12% trisodium phosphate, or spray washed with water at 35°C or 74°C (20.7 bar), in an automated spray-washing cabinet for 12 s, after 0, 2, or 4 h of exposure to the inoculated fecal paste. The samples were analyzed for streptomycin-resistant bacterial counts. Washing or washing/rinsing at time 0 (immediately after exposure to the inoculated fecal paste) with trisodium phosphate, 35°C water, hydrogen peroxide, acetic acid, and 74°C water removed 3.04 ± 0.40, 3.52 ± 0.55, 3.62 ± 0.67, 3.69 ± 0.72 and 4.17 ± 0.55 log CFU/cm2, respectively. Applying the spray-washing treatments 2 or 4 h after exposure to the fecal paste resulted in significantly (P < 0.05) less removal (1.76 to 3.89 log CFU/cm2 after 2 hand 0.94 to 2.58 Jog CFU/cm2 after 4 h) of bacteria. Thus, the time of exposure to fecal contamination affected the attachment of bacteria to beef-carcass tissue, as indicated by the decreasing numbers of bacteria removed by spray washing with increasing time of exposure, regardless of the washing treatment. The most effective washing agent was 74°C water at all washing times.

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