Isolations of parvovirus from cattle have not been reported since the isolation of bovine parvovirus 1 in 1959, although serologic surveys indicated that parvoviruses frequently infect cattle. Fecal and intestinal samples from calves with enteric diseases were inoculated onto bovine fetal lung (BFL) cells, bovine fetal kidney (BFK) cells, and cultures of a monkey kidney-cell line. Virus was isolated in 33 cases. Ten of these isolates were identified as bovine parvoviruses, and the remaining isolates were tentatively classified as bovine enteroviruses. BFL cells were more sensitive than BFK cells for the isolation of bovine parvoviruses. Two bovine parvoviruses were recovered from mixed .infections with enterovirus by reculture of the samples in the presence of antiserum to enterovirus. The parvovirus isolates were characterized on the basis of hemagglutination and hemadsorption of guinea-pig and human type O blood cells, development of Cowdry type A, Feulgen-positive intranuclear inclusions (which stained with fluorescein-isothiocyanate-labeled antibodies to bovine parvovirus 1), the effect of bovine antiserum to parvovirus on HA and infectivity, and the inhibition of replication by 5-bromo-2′-deoxyuridine.