Hybridization of Variants of Chloramphenicol Acetyltransferase Specified by fi + and fi - R Factors
- 1 October 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (10) , 3049-3053
- https://doi.org/10.1073/pnas.69.10.3049
Abstract
An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.Keywords
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