Three-Dimensional Digital Confocal Raman Microscopy
- 1 January 1993
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 47 (1) , 75-79
- https://doi.org/10.1366/0003702934048497
Abstract
We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging.Keywords
This publication has 16 references indexed in Scilit:
- High-Fidelity Raman Imaging Spectrometry: A Rapid Method Using an Acousto-Optic Tunable FilterApplied Spectroscopy, 1992
- Confocal Raman Microspectroscopy: Theory and Application to Thin Polymer SamplesApplied Spectroscopy, 1992
- Multispectral Hadamard Transform Raman MicroscopyApplied Spectroscopy, 1991
- A compact Raman microprobe/microscope: Analysis of polydiacetylene Langmuir and Langmuir‐Blodgett filmsMakromolekulare Chemie. Macromolecular Symposia, 1991
- Description and performance of a highly sensitive confocal Raman microspectrometerJournal of Raman Spectroscopy, 1991
- Studying single living cells and chromosomes by confocal Raman microspectroscopyNature, 1990
- Image restoration in 3-D microscopy with limited dataPublished by SPIE-Intl Soc Optical Eng ,1990
- Solution of Toeplitz systems for the restoration of 3-D optical-sectioning microscopy dataPublished by SPIE-Intl Soc Optical Eng ,1990
- Handbook of Biological Confocal MicroscopyPublished by Springer Nature ,1990
- Line‐scanned micro Raman spectroscopy using a cooled CCD imaging detectorJournal of Raman Spectroscopy, 1990