Mutation Spectra of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants
Open Access
- 1 June 2002
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (11) , 5822-5828
- https://doi.org/10.1128/jvi.76.11.5822-5828.2002
Abstract
To examine whether the exonuclease activity intrinsic to the polymerase (Pol) of herpes simplex virus type 1 can influence the mutational spectra, we applied the denaturing gradient gel electrophoresis (DGGE) system combined with sequencing to characterize thymidine kinase mutants isolated from both the wild-type virus and a mutant deficient in exonuclease activity, Y7. Wild-type viruses produced predominately frameshift mutations (67%), whereas Y7 replicated a significantly lower proportion of frameshifts (21%; P < 0.005). Furthermore, the majority of substitutions were transitional changes in both groups, although they distributed differently. The implications of these findings are discussed.Keywords
This publication has 16 references indexed in Scilit:
- Replication Fidelity of the supF Gene Integrated in the Thymidine Kinase Locus of Herpes Simplex Virus Type 1Journal of Virology, 2002
- The Fidelity of Human DNA Polymerase γ with and without Exonucleolytic Proofreading and the p55 Accessory SubunitJournal of Biological Chemistry, 2001
- DNA Replication FidelityAnnual Review of Biochemistry, 2000
- Mutations in the Exo III Motif of the Herpes Simplex Virus DNA Polymerase Gene Can Confer Altered Drug SensitivitiesVirology, 1998
- An altered spectrum of herpes simplex virus mutations mediated by an antimutator DNA polymeraseGene, 1995
- A net +1 frameshift permits synthesis of thymidine kinase from a drug-resistant herpes simplex virus mutant.Proceedings of the National Academy of Sciences, 1994
- Biochemical Basis of DNA Replication FidelityCritical Reviews in Biochemistry and Molecular Biology, 1993
- [4] Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acidsPublished by Elsevier ,1992
- Generation of genetic diversity in herpes simplex virus: An antimutator phenotype maps to the DNA polymerase locusVirology, 1984
- Frameshift Mutations and the Genetic CodeCold Spring Harbor Symposia on Quantitative Biology, 1966