CEREBRAL ISCHAEMIA IN THE RAT: INCREASED PERMEABILITY OF POST‐SYNAPTIC MEMBRANES TO HORSERADISH PEROXIDASE IN THE EARLY POST‐ISCHAEMIC PERIOD

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Abstract
An earlier study of the rat hippocampal stratum radiatum after transient cerebral ischaemia has shown cell membrane breaks, mainly postsynaptically, occurring as early as 20 min after an ischemic episode. In this study HRP [horseradish peroxidase] was injected into the lateral ventricle 10-15 min ischemia and allowed to diffuse until 60 min post-ischemia. Ultrastructural examination in the control animals, showed that HRP was localized exclusively in the extracellular space. After 10 min of transient ischemia, HRP was not confined to the extracellular space, but was also seen in .apprx. 10% of apical dendrites. Only a very few pre-synaptic terminals showed the presence of HRP. There is evidence of early post-ischemic membrane damage occurring in vivo in the apical dendrites of the CA-1 pyramidal cells.