Determination of Benzodiazepines in Serum by High Performance Liquid Chromatography Using Radially Compressed Columns and an Aqueous Methanolic Mobile Phase
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (6) , 549-557
- https://doi.org/10.1080/00032718208064399
Abstract
Diazepam, oxazepam and N-demethyldiazepam were determined by high performance liquid chromatography using a radially compressed C18 column and an aqueous methanolic mobile phase. The chromatographic separation was completed within 10 min. The drugs were recovered from [human] serum by extraction with hexane:ethyl acetate 70:30, v/v [vol/vol]. The method was linear in the range 50-1600 ng/ml for all drugs. Coefficients of variation were < 6.2% for 2 studied concentration levels.This publication has 7 references indexed in Scilit:
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