Characterization of the preformed antibodies involved in the xenograft reaction

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Abstract
The dog‐to‐sheep renal xenograft model was utilized to isolate the antibodies that participate in xenograft hyperacute rejection. These sheep antibodies, which were isolated from the rejected dog kidney, agglutinated and killed only dog blood and kidney cells whereas the whole sheep serum reacted with both dog and rabbit cells. The purified antibody was also utilized to obtain in pure form the antigens involved in the hyperacute rejection. When this antigen was tested for its ability to bind the serum of various species, this particular antigen was highly specific for sheep serum and would not react with rabbit, horse, calf, or mouse sera.